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Mission StatementDiapharma Group, Inc. in West Chester, Ohio sells hemostasis, thrombosis, platelet function testing, instrumentation, and apoptosis products in the diagnostic and research fields and provides strong technical competence and experienc...
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Diapharma/Chromogenix S-2765™/S821413/Vial/25mg

Diapharma/Chromogenix S-2765™/S821413/Vial/25mg
  • Diapharma/Chromogenix S-2765™/S821413/Vial/25mg
商品介绍

Description:

ChromogenixS-2765™isachromogenicsubstratefordeterminationofFactorXaactivity.Itisalsoverysensitivetotrypsin.

S-2765™ issuitable formeasuringFXainhibition inheparinanti-Xaassaysandantithrombinanti-Xaassays.

Composition:

EachvialcontainsthechromogenicsubstrateS-2765™,25mgandmannitol60mgasabulkingagent.

StABIlity:Lyophilizedsubstance:stableat25°Cuntilexpirydateprintedonthelabel.Thesubstanceishygroscopicandshouldbestoredinadryplace.

Solution:2mmol/LinH2Oisstableforsixmonthsat2to8°C.Suitablestocksolution:1-2mmol/LinH2O.

Chemicalname:N-a-Benzyloxycarbonyl-Darginyl-L-glycyl-L-arginine-pnitroaniline-dihydrochloride

Formula:N-a-Z-D-Arg-Gly-Arg-pNA·2HCl

Mol.wt.:714.6

chromogenix chromogenic substrate assay test kit

FactorXaIUandEnzymeActivity:

FactorXa,whichhasamolecularweightof44KDa,istheactivatedformofFactorX(MW:59KDa).

TheInternationalUnitsofFactorXcorrespondtotheamountofFactorXcontainedin1mlofnormalplasma.Thisisabout8mg/lor0.13µmol/l.

SincethereisnoWHOstandardforFXa,onewouldassumethatifalltheFactorXinnormalplasmawasconvertedtotheactivatedform,theFactorXaconcentrationwouldbeapproximately5.7mg/l.

TheactivityofhumanFactorXaascalculatedfromthekinetictablesis1.5nkat/µgwiththechromogenicsubstrateS-2222™,and4.4µkat/µgwiththechromogenicsubstrateS-2765™.

Theactivityof1µgofFactorXaasdeterminedbyFrieberger(1)is1.9nkatchromogenicsubstrateS-2222™.

Thus,1plasmaequivalentunitofFactorXwouldcorrespondto15.2nkatchromogenicsubstrateS-2222™.

FribergerPetal.
Syntheticpeptidesubstrateassaysandfibrinolysisandtheirapplicationonautomates.In:SeminarsinThrombosisandHaemostasis,Vol.9,281-300(1983).

FactorXMethod:

DeterminationoffactorXinplasmawithChromogenicSubstrateS-2765™

MeasurementPrinciple

Themethodisbasedonatwo-stageprinciple.Instageone,FactorXisactivatedinthepresenceofcalciumtoFactorXa(FXa)usingtheactivatorRussell’sVipervenom(RVV).Instagetwo,thegeneratedFXahydrolysesthechromogenicsubstrateZ-D-Arg-Gly-Arg-pNA(S-2765™),thusliberatingthechromophoricgrouppNA(p-nitroaniline).Thecoloristhenreadphotometricallyat405nm.ThegeneratedFXa(andthustheintensityofcolor)isproportionaltotheFXactivityofthesample.

FactorXRVV
FactorXa
Z-D-Arg-Gly-Arg-pNA+H2OFXa
Z-D-Arg-Gly-Arg-OH+pNA

Reagents

  1. S-2765™,25mgArt.No.S821413
    ReconstitutethesubstrateS-2765™(MW:714.6)with20mlsterilewater.
  2. Russell’sViperVenom(RVV)
    PrepareasolutionofRussell’sViperVenomataconcentrationof0.087mg/ml.
  3. CaCl2
    0.1mol/lcalciumchloridesolution.
  4. TrisEDTABuffer
    Dilutethebuffer1:10withdistilledwateraccordingtotheinsertsheetinstructions.
  5. NormalPlasma
    Calibrated,lyophilizedorfreshfrozenhumanplasmaisusedforthestandardizationoftheassay.Apoolednormalplasmacanbepreparedbytakingsamplesfrom20healthydonors.10-30mlcitrateblood(9volbloodand1vol0.1mol/lsodiumcitrate)fromeachdonoriscentrifugedat2000xgfor20minutesat15-25°C.Theplasmaispooledandsubsequentlydispensedinsmallvolumes,whicharefrozenrapidlyat-20°Corbelow.Toavoidlowtemperatureactivationofprekallikreintheplasmaiskeptat15-25°Cbeforeuseorfreezing.Thawingofplasmashouldbeperformedat37°Candthenkeptat15-25°Cuntilused.
  6. RVV+CaCl2
    Beforeuse,mix1volumeofRVVwith1volumeofCaCl2.Themixtureisstablefor48hoursat2-8°C.
  7. Aceticacid20%
    Aceticacidisusedasastoppersolutionintheend-pointmethod.

Specimencollection

Blood(9vol)ismixedwith0.1mol/lsodiumcitrate(1vol)andcentrifugedat2000xgfor20minutesat20-25°C.Storage:1weekat2-8°Cor3-4monthsat-20°C.


Standardcurve

Predilution

FinalDilution

FX%NormalPlasma mlBuffer mlPredilplasma mlBuffer ml
01000
252575501000
505050501000
757525501000
100501000
12450800

Method

SampleDilution
Buffer1000
Testplasmaorstandard50
Mix

AcidStoppedMethodAB
DilutedSample200 ml50 ml
Incubateat37°C3-4min3-4min
Substrate(37°C)200 ml50 ml
Mixandaddwithin30sec
RVV+ CaL2200 ml50 ml
Mixandincubateat37°C3min3min
Aceticacid20%200 ml50 ml

A=testtubemethod
B=microplatemethod

Sampleblankactivitiesshouldbedeterminedandsubtractedwhenanalyzingstronglycoloredplasma,e.g.lipemicandhemolytic.Thesampleblanksarepreparedbymixingthedilutedsample,aceticacid20%andwaterinsteadofthereagents(400µlfortesttubesand100µlformicroplates).Readtheabsorbanceofthesamplesandblanksat405nm.Thecolorisstableforatleastfourhours.WhenpossIBLe,useadualwavelengthmodewith490nmasthereferencewavelength.


Initialratemethod

Whenperformingtheinitialratemethod,transferthemicroplatetoamicroplatereaderimmediatelyaftertheadditionofRVV+CaCl2andreadthechangeinA/min.Themicroplatereadermustbepre-incubatedat37°C.


Calculation

PlotAorΔA/minforthestandardsagainsttheirconcentrationofFactorX.ReadtheFactorXvalueforthecorrespondingAorΔA/minoftheunknowntestsamplefromthestandardcurve.


Bibliography

  1. KieselWetal.FactorXactivatingenzymefromRussell’sViperVenom;isolationandcharacterisation.Biochemistry15,4901-4905(1976).
  2. LindhoutMJetal.ActivationofdecarboxyfactorXbyaproteinfromRussell’sViperVenom.PurificationandpartialcharacterisationofactivateddecarboxyfactorX.BiochemBiophysActa533,327-341,(1978).
  3. BergströmKandEgbergN.DeterminationofvitaminKsensitivecoagulationfactorsinplasma.Studiesonthreemethodsusingsyntheticchromogenicsubstrates.ThrombRes12,531-547(1978).
  4. VanWijkEMetal.ArapidmanualchromogenicfactorXassay.ThrombRes22,681-686(1981).EgbergNandHeedmanPA.SimplifiedperformanceofamidoliticfactorXassay.ThrombRes25,437-440(1982).
  5. ChabbatJetal.AprotininisacompetitiveinhibitorofthefactorVIIa-tissuefactorcomplex.ThrombRes71,205-215(1993).
  6. MielickiWPandGordonSG.ThreestagechromogenicassayfortheanalysisofactivationpropertiesoffactorXbycancerprocoagulant.BloodCoagulFibrinol4,441-446(1993).
  7. KoppakaVetal.SolublephospholipidsenhancefactorXa-catalyzedprothrombinactivationinsolution.Biochemistry35,7482-7491(1996).
  8. RiesbeckKetal.HumantissuefactorpathwayinhibitorfusedtoCD4bindsbothFXaandTF/FVIIaatthecellsurface.ThrombHaemost78,1488-1494(1997).
  9. RomischJetal.Comparativeinvitroinvestigationofprothrombincomplexconcentrates.SeminThrombHemost24,175-181(1998)
  10. FariaF,etal.AnewfactorXainhibitor(lefaxin)fromtheHaementeriadepressaleec.ThrombHaemost82,1469-73(1999).
品牌介绍

Mission Statement

Diapharma Group, Inc. in West Chester, Ohio sells hemostasis, thrombosis, platelet function testing, instrumentation, and apoptosis products in the diagnostic and research fields and provides strong technical competence and experience to ensure customer expectations will be met or exceeded.

diapharma hemostasis chromogenic clotting elisa assay test kit

 

History

The Diapharma Group, Inc. formed on January 1, 1997 from Pharmacia Hepar, Inc. in Franklin, Ohio, and began as the exclusive US and Canadian distributor of the Chromogenix substrates and assays.

Over a quarter-century ago, Chromogenix developed the first chromogenic substrate technology under the former name, Kabi Diagnostica. Kabi later merged with Pharmacia. Some of Diapharma’s current staff worked inside the Chromogenix department within Pharmacia’s heparin manufacturing plant.

In 1998, Diapharma moved to West Chester, Ohio, where it remains today. Over the years, Diapharma expanded its product line to include a variety of hemostasis, cell death, platelet function, ecotoxicology, assays, reagents, antibodies and instruments from superior manufacturers.

In 2017, Diapharma celebrated twenty years of success



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Diapharma Group,Inc


Diapharma Group INC成立于1997年,研究人员具有多年临床以及研发经验,专注于血液病领域的试剂盒,试剂以及仪器,主要涵盖血栓形成、止血、血小板功能分析、细胞凋亡、生态毒理学等。


The Diapharma Group, Inc. formed on January 1, 1997 from Pharmacia Hepar, Inc. in Franklin, Ohio, and began as the exclusive US and Canadian distributor of the Chromogenix substrates and assays.


Over a quarter-century ago, Chromogenix developed the first chromogenic substrate technology under the former name, Kabi Diagnostica. Kabi later merged with Pharmacia. Some of Diapharma’s current staff worked inside the Chromogenix department within Pharmacia’s heparin manufacturing plant.


In 1998, Diapharma moved to West Chester, Ohio, where it remains today. Over the years, Diapharma expanded its product line to include a variety of hemostasis, cell death, platelet function, ecotoxicology, assays, reagents, antibodies and instruments from superior manufacturers.


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DiaPharma Group INC

Peviva M30-Apoptosense®*

ELISA

Kit/96well  P10011

DiaPharma Group INC

Peviva M30 CytoDEATH™*

ELISA

Kit/96well P10900

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Peviva M30 CytoDEATH™, MAb*

Antibody

200tests P10700

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Peviva M30 CytoDEATH™, MAb Biotinylated*

Antibody

200tests P10750

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Peviva M30 CytoDEATH™, MAb Fluorescein*

Antibody

200tests P10800

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Peviva M30 CytoDEATH™, MAb Orange*

Antibody

200tests P10830

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Peviva M65® ELISA*

ELISA

Kit/96well  P10020

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Peviva M65 EpiDEATH® ELISA*

ELISA

Kit/96well  P10040

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Peviva M5 CytoKERATIN™ 18, MAb*

Antibody

200tests P10600

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Peviva M6 CytoKERATIN™ 18, MAb*

Antibody

200tests P10650

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TECO® Intact Proinsulin* NEW

ELISA

Kit/96well TE1011

DiaPharma Group INC

TECO® Adiponectin High Sensitive* NEW

ELISA

Kit/96well TE1014

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TECO® Leptin* NEW

ELISA

Kit/96well TE1016

DiaPharma Group INC

TECO® Hyaluronic Acid (HA) Plus* NEW

ELISA

Kit/96well TE1018-2

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REAADS Hyaluronic Acid (HA)*

ELISA

Kit/96well K029-001

DiaPharma Group INC

TECO® Mucus Collection Set* NEW

ELISA

Kit TE1034

DiaPharma Group INC

TECO® Perch Vitellogenin* NEW

ELISA

Kit/96well  TE1035

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TECO® Cyprinid Vitellogenin* NEW

ELISA

Kit/96well TE1037

DiaPharma Group INC

Chromogenix S-2222™*

FXa

25mg S820316

DiaPharma Group INC

Chromogenix S-2238™*

Thrombin

25mg S820324

DiaPharma Group INC

Diapharma CS TH*

Thrombin

5mg** DPG238-5

DiaPharma Group INC

Chromogenix S-2251™*

Plasmin, Plasminogen-SK

25mg S820332

DiaPharma Group INC

Chromogenix S-2288™*

t-PA, other Serine Proteases

25mg S820852

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Chromogenix S-2302™*

Kallikrein, FXIa, FXIIa

25mg S820340

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Diapharma CS GK* NEW

Glandular Kallikrein

25mg DPG266-25

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Chromogenix S-2366™*

Activated Protein C, FXIa

25mg S821090

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Chromogenix S-2403™*

Plasmin, Plasminogen-SK

25mg S822254

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Diapharma CS UK*

Urokinase

25mg DPG444-25

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Diapharma CS PSA (KLK3)*

Chymotrypsin, PSA Enzyme Activity

25mg DPG586-25

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Technoclone Siron LS (aPTT liquid)*


2x4ml 5035105

DiaPharma Group INC

Technoclone Siron LS (aPTT liquid)*


10x4ml 5035107

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Technoclone Siron LS (aPTT liquid)*


10x10ml 5035109

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Technoclone Siron (aPTT liquid)*


2x4ml 5035118

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Technoclone Siron (aPTT liquid)*


10x4ml 5035119

DiaPharma Group INC

Technoclone Siron (aPTT liquid)*


10x10ml 5035121

DiaPharma Group INC

Technoclot® PT Owren (manual) ~160T* NEW

Clotting

10x4ml 5005032

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Technoclot® PT Owren (manual) ~400T* NEW

Clotting

10x10ml 5005037

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Technoclot® PT Owren (automated) ~800T* NEW

Clotting

10x4ml 5005044

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Technoclot® PT Owren (automated) ~2000T* NEW

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10x10ml 5005046

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Technoclot® PT Owren Capillary Calibrator Set* NEW

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4x1ml 5005100


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