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Diapharma/REAADS Anti-Cardiolipin (aCL) IgG/IgM/K023-001/Kit/96 tests

  • 产品编号: K023-001
  • 美  元  价: $0.00
  • 会  员  价: 待定
  • 品       牌: Diapharma
  • 产       地: 美国
  • 公       司: Diapharma Group, Inc.
  • 产品分类: ref="." class="index">首页 > Diapharma/REAADS Anti-Cardiolipin (aCL) IgG/IgM/K023-001/Kit/96 tests
  • 公司分类: Hemostasis Autoimmunity
:Kit/96 tests
Diapharma/REAADS Anti-Cardiolipin (aCL) IgG/IgM/K023-001/Kit/96 tests
  • Diapharma/REAADS Anti-Cardiolipin (aCL) IgG/IgM/K023-001/Kit/96 tests
商品介绍

Description:

REAADS Anti-Cardiolipin (aCL) IgG/IgM is an ELISA test kit for the determination of IgG/IgM anti-cardiolipin antibodies in human serum or plasma in individuals with systemic lupus erythematosus (SLE) and lupus-like disorders (anti-phospholipid syndrome).

Kit Composition:

Reagents, Storage and StABIlity

  • StABIlized beef heart cardiolipin (diphosphatidyl glycerol) coated microwells: 96 (12 strips of 8 breakaway wells), with frame.
  • Sample Diluent: 1 bottle (60 ml) (green solution), contains bovine calf serum. Contains sodium azide.
  • aCL IgG Calibrator Sera (1-high, 2-moderate, 3-low) (human): 3 vials (0.250 ml) see vial label for antibody concentration in GPL units. Calibrator 2 should be used when performing single point calibration. Contains sodium azide.
  • aCL IgM Calibrator Sera (1-high, 2-moderate, 3-low) (human): 3 vials (0.250 ml) see vial label for antibody concentration in MPL units. Calibrator 2 should be used when performing single point calibration. Contains sodium azide.
  • aCL IgG Positive Control Serum (human): 1 vial (0.250 ml) see vial label for expected GPL range. Contains sodium azide.
  • aCL IgM Positive Control Serum (human): 1 vial (0.250 ml) see vial label for expected MPL range. Contains sodium azide.
  • aCL Normal Control Serum (human): 1 vial (0.250 ml) see vial label for expected GPL and MPL ranges. Contains sodium azide.
  • anti-human IgG (goat) HRP-Conjugated Antibody Solution: 1 bottle (15 ml) (blue solution).
  • anti-human IgM (goat) HRP-Conjugated Antibody Solution: 1 bottle (15 ml) (red solution).
  • One Component Substrate (TMB and H2O2): 1 bottle (15 ml) ready to use.
  • Stopping Solution: 1 bottle (15 ml) (0.36 N sulfuric acid).
  • Wash Concentrate: 2 bottles (30 ml)  (33X PBS).

Store at 2 – 8°C. Do Not Freeze.

Measurement Principle:

The test is performed as an indirect ELISA. Diluted serum samples, calibrator sera, and controls are incubated in cardiolipin coated microwells, allowing aCL antibodies present in the samples to react with the immobilized antigen. After the removal of unbound serum proteins by washing, antibodies specific for human IgG or IgM labeled with horserADIsh peroxidase (HRP) are added forming complexes with the cardiolipin bound antibodies. Two enzymeconjugated antibody solutions are provided, one specific for human IgG antibodies and one specific for human IgM antibodies. The concentration of IgG aCL antibodies and IgM aCL antibodies must be determined separately. Following another washing step, the bound enzyme-antibody conjugate is assayed by the addition of tetramethylbenzidine (TMB) and hydrogen peroxide (H2O2) as the chromogenic substrate. Color develops in the wells at an intensity proportional to the serum concentration of aCL antibodies. Results are obtained by reADIng the O.D. (optical density or absorbance) of each well with a spectrophotometer. Calibrator sera are provided, with the IgG and IgM aCL concentrations expressed in GPL or MPL units, respectively. These units are traceable to the internationally recognized preparations of the Phospholipid Standardization Laboratory, University of Louisville. The user has the option of running either a single point calibrator or a four-point calibration curve. For single point calibration, dividing the concentration value of the calibrator sera by the O.D. value of the calibrator provides a conversion factor (one for IgG and one for IgM aCL). The O.D. values of all the other samples are multiplied by the conversion factors to obtain IgG and IgM aCL antibody concentrations in standard units. One GPL unit is equivalent to 1 µg/mL of an affinity purified standard IgG sample, and one MPL unit is equivalent to 1 µg/mL of an affinity purified standard IgM sample. For multipoint calibration, perform a linear regression analysis with calibrator values against calibrator O.D.s. Control and patient results are determined from the calibration curve.

Kit Sizes:

CATALOG #

TYPE

WELLS

FORMAT

K023-001 IgG / IgM 96 rapid format (15-15-10)
K023-002* IgG / IgM 288 rapid format (15-15-10)
K026-001 IgA 96 rapid format (15-15-10)
K026-006* IgA 288 rapid format (15-15-10)
K11139* IgG 96 extended incubations (30-30-30)
K12747* IgG 288 extended incubations (30-30-30)
K11140* IgM 96 extended incubations (30-30-30)
K12748* IgM 288 extended incubations (30-30-30)
K11141* IgA 96 extended incubations (30-30-30)
K12770* IgA 288 extended incubations (30-30-30)

* This product may require a special order. Please inquire at info@Diapharma.com or 1-800-526-5224.

Background:

Anti-cardiolipin (aCL) antibodies are associated with the presence of both venous and arterial thrombosis, thrombocytopenia, and recurrent fetal loss. These autoantibodies are frequently found in patients with systemic lupus erythematosus (SLE) and other autoimmune diseases, as well as in some individuals with no apparent previous underlying disease.

品牌介绍

Mission Statement

Diapharma Group, Inc. in West Chester, Ohio sells hemostasis, thrombosis, platelet function testing, instrumentation, and apoptosis products in the diagnostic and research fields and provides strong technical competence and experience to ensure customer expectations will be met or exceeded.

diapharma hemostasis chromogenic clotting elisa assay test kit

 

History

The Diapharma Group, Inc. formed on January 1, 1997 from Pharmacia Hepar, Inc. in Franklin, Ohio, and began as the exclusive US and Canadian distributor of the Chromogenix substrates and assays.

Over a quarter-century ago, Chromogenix developed the first chromogenic substrate technology under the former name, Kabi Diagnostica. Kabi later merged with Pharmacia. Some of Diapharma’s current staff worked inside the Chromogenix department within Pharmacia’s heparin manufacturing plant.

In 1998, Diapharma moved to West Chester, Ohio, where it remains today. Over the years, Diapharma expanded its product line to include a variety of hemostasis, cell death, platelet function, ecotoxicology, assays, reagents, antibodies and instruments from superior manufacturers.

In 2017, Diapharma celebrated twenty years of success


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