Diapharma/Multiplate® Prostaglandin E1/MP0160/Kit/1ml + 5 micro test tubes

Description:

DISCONTINUED–REPLACEMENT:MP0260

Multiplate®ProstaglandinE1(PGE1)isareagentkitfor theassessmentofADPtestHSand assessmentofpositive(i.e.abnormal) controlsoftheADPtest ontheMultiplate®instrument. Fortheevaluationofqualitativeplateletdefectsaswellas plateletfunctioninhibition.PGE1enhancesthesensitivity ofADPtesttoplateletfunctioninhibitionespeciallytothe effectofclopidogrel.Moreover,theadditionofahigher doseofPGE1intothesamplecaninduceanabnormal aggregationinADPtest,whichallowstheperformanceof anabnormalcontrol(positivecontrol).ProstaglandinE1(PGE1)isusedincombinationwiththe ADPtestreagent(MP0120).IntheADPtestHS,anincompleteblockadeofaggregationisoftenseenwithclopidogreltherapy.ThismaybeduetothefactthatADPnotonlytriggerstheP2Y12 receptorontheplateletsurface(e.g.,thereceptorthatisblockedbyclopidogrel),butalsootherADPreceptors(whicharenotaffectedbycopidogrel).AcombinationofADPandphysiologicalplateletinhibitor(prostaglandinE1(PGE1))canbemoresensitiveforthedetectionoftheactionofclopidogrelthantheuseofADPalone.ThebindingofADPtotheP2Y12receptorreducesthelevelofcAMPintheplatelet,whichinturnenhancesthereleaseofcalciumfromendogenoussources.Theenhancementofintracellularcalciumleadstotheactivationandaggregationoftheplatelet.PGE1reducesthemobilizationofcalciumandthusinhibitsplateletaggregation.AsclopidogrelalsoreducestheplateletactivationbyADP,clopidogrelandPGE1actsynergistically.

KitComposition:

Reagents,StorageandStABIlity

• MP0160:Multiplate®ProstaglandinE1, PGE1,Kit,1x1.0mL, lyophilized(300nM),with5microtesttubesfor aliquotation. An unopenedreagentisstableuntiltheexpirydateprinted ontheviallabelwhenstoredat 2-8°C, 7daysafterreconstitutionwhenstoredat 2-8°C,4weeks <-20°C,or 24hoursatroomtemperatureafterone timethawing.

AlsoAvailable

• MP0096: Multiplate®PGtestAliquotVials,Bag,100microtubescolorcodedmicrotesttubesfor aliquotation.
• MP0260:Multiplate®ProstaglandinE1, PGE1, Kit,3x1.0mL, lyophilized(300nM),withoutmicrotesttubesfor aliquotation.
• MP0540:Multiplate®ProstaglandinE1, PGE1, Bag,1x1.0mL,lyophilized(300nM),withoutmicrotesttubesfor aliquotation.

MeasurementPrinciple:

PGE1isanaturalplateletinhibitorwhichtriggersan increaseincAMPlevelsintheplatelet.cAMPisasocalled secondmessenger,i.e.anintracellularsignalling molecule.AdecreaseofthecAMPlevelintheplatelet leadstoplateletactivation.AnincreaseofthecAMPlevel counteractsplateletactivation. Theadditionof20µlPGE1totheADPtest(9.4nMPGE1 finalconc.)inducesamoderateinhibitionofplatelet activationinhealthynormalbloodsamples,buta significantincreaseofsensitivityoftheADPtesttothe plateletinhibitionbyclopidogrel. Theadditionof50µlPGE1intoADPtest(22nMPGE1 finalconc.)normallyinducesastronginhibitionofADP inducedaggregation(positivecontrolforADPtest).

Mission Statement

Diapharma Group, Inc. in West Chester, Ohio sells hemostasis, thrombosis, platelet function testing, instrumentation, and apoptosis products in the diagnostic and research fields and provides strong technical competence and experience to ensure customer expectations will be met or exceeded.

diapharma hemostasis chromogenic clotting elisa assay test kit

History

The Diapharma Group, Inc. formed on January 1, 1997 from Pharmacia Hepar, Inc. in Franklin, Ohio, and began as the exclusive US and Canadian distributor of the Chromogenix substrates and assays.

Over a quarter-century ago, Chromogenix developed the first chromogenic substrate technology under the former name, Kabi Diagnostica. Kabi later merged with Pharmacia. Some of Diapharma’s current staff worked inside the Chromogenix department within Pharmacia’s heparin manufacturing plant.

In 1998, Diapharma moved to West Chester, Ohio, where it remains today. Over the years, Diapharma expanded its product line to include a variety of hemostasis, cell death, platelet function, ecotoxicology, assays, reagents, antibodies and instruments from superior manufacturers.

In 2017, Diapharma celebrated twenty years of success