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Diapharma/TECO® Mucus Collection Set/TE1034/Kit

  • 产品编号: TE1034
  • 美  元  价: $0.00
  • 会  员  价: 待定
  • 品       牌: Diapharma
  • 产       地: 美国
  • 公       司: Diapharma Group, Inc.
  • 产品分类: ref="." class="index">首页 > Diapharma/TECO® Mucus Collection Set/TE1034/Kit
  • 公司分类: Ecotoxicology
:Kit
Diapharma/TECO® Mucus Collection Set/TE1034/Kit
  • Diapharma/TECO® Mucus Collection Set/TE1034/Kit
商品介绍

Description:

The TECO® Mucus Collection Set is a collection set for fish mucus, including validated swabs, sample tubes in a sample tube rack, a specifically designed extraction buffer and a kit instruction. The Mucus Collection Set is a validated tool for repeatable non-invasive/non-destructive sampling of epidermal mucosa for vitellogen determination.

The TECO® Mucus Collection Set for fish mucus is a validated tool for repeatable non-destructive sampling of epidermal mucosa for vitellogenin determination using the:

  • TECO® Perch (Perciformes) Vitellogenin ELISA Kit, TE1035
  • TECO® Cyprinid Vitellogenin ELISA Kit, TE1037
    Components are ready to use.

Kit Composition:

1: Breakable Swabs, 42. Ready to use.
2: Reaction Vials, 42. Ready to use.
3: Extraction Buffer, 1 x 25 ml Ready to use.
4: Rack for Reaction Vials, 1 x
I: Kit instruction, 1 x

Background:

Vitellogenin (VTG) is one of the core endpoints in screening and testing for endocrine disrupting chemicals standardized in the OECD Guidelines for the testing of chemicals for estrogenic activity. Originally believed to be produced only in the liver, several cell types have recently been shown to produce VTG after estrogen stimulation, including those of the epidermal mucosa of fish.

Given the destructive nature of trADItional sampling, collecting successive samples from fish has been a widespread problem, as for instance in the analysis of vitellogenin induction while screening and testing chemical substances for endocrine disruption potential. Blood is difficult to collect, in particular where very small fish are concerned, or in approaches where the animals must survive sampling. This is particularly important in field monitoring in order to avoid impact on the population under investigation.

Even though the VTG concentration in the skin mucus is an order of magnitude lower than in blood serum or in body homogenates (containing liver tissue), the skin mucosa is very well suited as a matrix to determine exogenous VTG induction caused by environmental chemicals with affinity to the estrogen receptors. Mucus sampling with the aid of swabs constitutes an alternative to trADItional procedures, avoiding animal injury by using a highly sensitive ELISA in combination with a high quality extraction buffer and swab sampling, the determination of mucosa-derived VTG has the following advantages:

  • Non-destructive, non-invasive sampling
  • Defined matrix without lymphatic fluid contamination
  • Allows the use of smaller fish for testing and monitoring purposes
  • Repeated sampling allows for individual recording of the induction kinetic

The sampling technique leads to highly comparable results when testing samples from the left and the right side from the same individual in inter-laboratory trials.

品牌介绍

Mission Statement

Diapharma Group, Inc. in West Chester, Ohio sells hemostasis, thrombosis, platelet function testing, instrumentation, and apoptosis products in the diagnostic and research fields and provides strong technical competence and experience to ensure customer expectations will be met or exceeded.

diapharma hemostasis chromogenic clotting elisa assay test kit

 

History

The Diapharma Group, Inc. formed on January 1, 1997 from Pharmacia Hepar, Inc. in Franklin, Ohio, and began as the exclusive US and Canadian distributor of the Chromogenix substrates and assays.

Over a quarter-century ago, Chromogenix developed the first chromogenic substrate technology under the former name, Kabi Diagnostica. Kabi later merged with Pharmacia. Some of Diapharma’s current staff worked inside the Chromogenix department within Pharmacia’s heparin manufacturing plant.

In 1998, Diapharma moved to West Chester, Ohio, where it remains today. Over the years, Diapharma expanded its product line to include a variety of hemostasis, cell death, platelet function, ecotoxicology, assays, reagents, antibodies and instruments from superior manufacturers.

In 2017, Diapharma celebrated twenty years of success


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